Boca Hydro Gibberellin GA₃ 90% – High Purity Plant Growth Regulator Powder for Rooting, Tissue Culture Media, Micropropagation, Seed Germination, Shoot Elongation, and Advanced Propagation Systems

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Categories: Plant Tissue Culture
Vendor: Boca Hydro LLC

Boca Hydro Gibberellin (GA₃) 90% is a high-purity plant growth regulator designed for professional horticulture, research, and tissue culture applications. Gibberellins are natural plant hormones that promote cell elongation, break seed dormancy, and stimulate uniform germination and vigorous growth.

This highly concentrated 90% GA₃ powder provides reliable performance in very small doses, making it ideal for tissue culture rooting media, shoot elongation, and seed germination enhancement. It dissolves easily when pre-mixed with ethanol or NaOH and is suitable for use in hydroponics, nurseries, laboratories, and advanced propagation systems.

  • High Purity Formula: Boca Hydro Gibberellin GA₃ is standardized to 90% purity for consistent, professional-grade results in plant science and horticulture.

  • Seed Germination & Dormancy Breaking: Enhances germination of difficult or slow-germinating seeds by breaking natural dormancy.

  • Rooting & Shoot Elongation: Widely used in plant tissue culture and micropropagation to promote rooting, elongation of shoots, and uniform plant development.

  • Versatile Applications: Suitable for hydroponic systems, seed treatments, nursery propagation, foliar sprays, and laboratory tissue culture media.

  • Easy to Prepare Stock Solutions: Easily dissolves in ethanol or NaOH before dilution; can be made into convenient stock solutions for precise dosing.

Dosing

For plant tissue culture, GA₃ (Gibberellic Acid) is normally used at very low concentrations — usually in the range of 0.1 to 5 mg/L depending on species and the purpose (shoot elongation vs rooting).

Step 1: Decide concentration

For rooting mixes, a common starting point is 0.1–1.0 mg/L.
Some woody plants may use up to 3–5 mg/L, but higher levels often inhibit rooting and cause excessive elongation.

Step 2: Convert mg/L to actual GA₃ powder weight

1 mg/L means 1 mg of active GA₃ per 1 L medium.
Since your powder is 90% pure, you must adjust:

Powder required (mg)=Desired GA₃ (mg)0.90\text{Powder required (mg)} = \frac{\text{Desired GA₃ (mg)}}{0.90}

Examples:

  • 0.1 mg/L → 0.11 mg of powder per liter

  • 1.0 mg/L → 1.1 mg of powder per liter

  • 5.0 mg/L → 5.6 mg of powder per liter

Step 3: Solubility note

GA₃ is poorly soluble in water. It should be first dissolved in a small drop of 95% ethanol or 1–2 drops of 1N NaOH, then diluted with sterile distilled water before adding to the media.

Practical recommendation for rooting medium:
Start with 0.5–1.0 mg/L GA₃ (≈0.55–1.1 mg of  90% GA₃ powder per liter of medium), unless you are following a crop-specific protocol.

Making a concentrated stock solution is the standard way to handle GA₃ so you don’t need to weigh out fractions of a milligram each time. Here’s how you can prepare it:

GA₃ (90%) Stock Solution Recipe

Target stock: 1 mg/mL (1000 mg/L)

  1. Weigh the powder:
    GA₃ required = 111 mg of 90% GA₃ powder
    (because 111 mg × 0.90 = ~100 mg active GA₃).

  2. Dissolve:
    Place powder in a small sterile container.
    Add 1–2 mL of 95% ethanol to dissolve completely.
    Once dissolved, bring the volume up to 100 mL with sterile distilled water.

  3. Sterilize:
    Filter-sterilize (0.22 µm syringe filter).
    Store in sterile screw-cap vials or bottles.

  4. Storage:
    Short-term (a few weeks): Keep at 39–46 °F (that’s 4–8 °C, a typical refrigerator).
    Long-term (months): Keep at –4 to –13 °F (that’s –20 to –25 °C, a standard lab freezer).
    Protect from light ( Cover with foil )

How to Use the Stock


Desired medium concentration: 0.5–1.0 mg/L GA₃
Stock: 1 mg/mL = 1000 mg/L

Volume to add (mL per 1 L media)=Desired conc (mg/L)1000\text{Volume to add (mL per 1 L media)} = \frac{\text{Desired conc (mg/L)}}{1000}


For 0.5 mg/L → 0.5 mL stock per L medium
For 1.0 mg/L → 1.0 mL stock per L medium

Add the stock after autoclaving and cooling the media to ~50 °C, under sterile conditions (e.g., laminar hood).