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$29.99
Boca Hydro Naphthaleneacetic Acid (NAA) 98%
Boca Hydro Naphthaleneacetic Acid (NAA) 98% is a high-purity synthetic plant growth regulator and auxin used to promote root initiation, cell division, and callus formation in tissue culture and propagation systems.
NAA is an important component in many Murashige & Skoog (MS)-based media and other micropropagation protocols where root induction, callus formation, and organogenesis are desired.
This fine, water-dispersible crystalline powder provides consistent performance in plant tissue culture, hydroponics, and advanced propagation applications. NAA functions similarly to natural indole-3-acetic acid (IAA) but offers significantly greater stability, making it well suited for storage and use in liquid or agar-based media.
Formulated for professional laboratory and research use, Boca Hydro NAA is compatible with many commonly used plant growth regulators, including BA, Kinetin, TDZ, GA₃, and IBA, allowing precise adjustment of auxin-to-cytokinin balance for rooting, callus development, and morphogenesis.
Typical Applications
| Application | Purpose |
|---|---|
| Tissue Culture Rooting | Supports root initiation and development in cultured plant material |
| Callus Formation | Used alone or with cytokinins to support callus induction |
| Stem & Leaf Explant Regeneration | Helps regulate organogenesis and regeneration response |
| Microshoot Rooting | Supports rooting of shoots produced through micropropagation |
| Cutting Propagation | Used as an auxin source for rooting propagation material |
| Auxin / Cytokinin Balance | Allows fine adjustment of plant growth regulator ratios in culture media |
NAA 98% Tissue Culture & Preparation Guide
Because NAA is only sparingly soluble in water, preparing a concentrated stock solution is recommended for accurate tissue culture dosing.
NAA should first be dissolved in a small amount of 95% ethanol or 1 N NaOH before being brought to final volume with distilled water.
Prepare a 1 mg/mL (1,000 ppm) Stock Solution
| Ingredient | Amount |
|---|---|
| Boca Hydro NAA 98% | 0.1 g (100 mg) |
| 95% Ethanol | 1–2 mL for initial dissolution |
| Alternative Solvent | A few drops of 1 N NaOH |
| Distilled Water | Bring to a final total volume of 100 mL |
| Target Stock Strength | 1 mg/mL (1,000 ppm) |
Stock Solution Preparation
- Measure 0.1 g (100 mg) of Boca Hydro NAA 98%.
- Add 1–2 mL of 95% ethanol or a few drops of 1 N NaOH.
- Mix until the NAA is fully dissolved.
- Add distilled water and bring to a final total volume of 100 mL.
- Transfer the finished stock solution to a suitable dark or light-protected bottle.
Finished Tissue Culture Media Dosing
Use the prepared 1 mg/mL NAA stock solution to dose MS media, basal media, agar media, or other tissue culture formulations.
| Application | Final NAA Concentration | 1 mg/mL Stock per 1 Liter Media |
|---|---|---|
| Low Root Induction | 0.1 mg/L (0.1 ppm) | 100 µL (0.1 mL) |
| Moderate Root Induction | 0.25 mg/L (0.25 ppm) | 250 µL (0.25 mL) |
| Higher Root Induction | 0.5 mg/L (0.5 ppm) | 500 µL (0.5 mL) |
| Callus Induction | 1.0 mg/L | 1 mL |
| Higher Callus Induction | 2.0 mg/L | 2 mL |
Typical Concentration Ranges
| Use | Typical NAA Range | General Purpose |
|---|---|---|
| Root Induction | 0.1–0.5 mg/L | Root initiation and development of cultured shoots |
| Callus Induction | 1–2 mg/L | Callus production, commonly in combination with cytokinins |
Using NAA with Other Plant Growth Regulators
NAA may be used with other plant growth regulators to adjust the hormonal balance of tissue culture media and influence rooting, callus formation, shoot development, and organogenesis.
| Plant Growth Regulator | Compatibility / Use |
|---|---|
| BA (6-Benzylaminopurine) | Auxin / cytokinin combinations for shoot and callus protocols |
| Kinetin | Used with NAA for cytokinin-to-auxin balance adjustment |
| TDZ | May be incorporated into advanced regeneration protocols |
| GA₃ | Compatible with protocols requiring gibberellin activity |
| IBA | May be combined or compared in rooting and auxin-response protocols |
When to Add NAA to Tissue Culture Media
- Prepare your MS, basal, agar, or liquid tissue culture medium.
- Adjust the medium to approximately pH 5.6–5.8 before sterilization.
- Autoclave the culture medium normally.
- Allow the sterilized medium to cool to approximately 50 °C.
- Add the required amount of prepared NAA stock solution.
- Mix gently and thoroughly under sterile conditions.
Important Tissue Culture Note
Sterilize the culture medium by autoclaving first, then add NAA stock after the medium has cooled to approximately 50 °C when following protocols that treat the plant growth regulator as a heat-labile component.
Stock Solution Storage
| Storage Condition | Recommendation |
|---|---|
| Temperature | 4 °C refrigerated |
| Light Exposure | Store in a dark or light-protected bottle |
| Typical Shelf Life | 6–12 months |
Recommended Media Conditions
| Parameter | Recommendation |
|---|---|
| Medium Type | MS, basal, agar, or liquid tissue culture media |
| Recommended pH | 5.6–5.8 before sterilization |
| Stock Addition | Add after autoclaving and cooling media to approximately 50 °C |
Compatible Boca Hydro Products
- Boca Hydro MS Medium
- Boca Hydro BA 98%
- Boca Hydro Kinetin 98%
- Boca Hydro IBA 98%
Quick Reference: For root induction, use approximately
0.1–0.5 mg/L NAA. For callus induction in combination with
cytokinins, approximately 1–2 mg/L NAA may be used according
to the specific plant culture protocol.
